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Projeto de pesquisa

  • Padrões de metabolismo planctônico na plataforma Sudeste Brasileira

  • Coordenador do projeto: Aurea Maria Ciotti  
  • Autor ou executor principal do projeto: Aurore Regaudie-de-Gioux  
  • Número do projeto: 892  
  • Categoria: Pós-doutorado 
  • Data de início das atividades no CEBIMar: 17/07/2014  
  • Data de término das atividades no CEBIMar: 13/07/2016  
  • Resumo: Coastal marine ecosystems are greatly threatened by anthropogenic global climate change and are the major focuses of concern for scientific community. The southeastern Brazilian shelf, arc-shaped sector of the southeastern Brazilian marging, is characterized by water mass dynamics strongly influenced by advances and retreats of the South Atlantic Central Water. The southeastern Brazilian shelf is a complex ecosystem and can be divided in three sectors: north of the São Sebastião Island characterized by land-derived organic matter inputs, south of the São Sebastião Island characterized by marine organic matter inputs and upwellings, and finally the channel of the São Sebastião Island characterized by strong wind fields and water current. The ecosystem of this continental shelf region may respond in different way to climate change considering its biological and physical complexities and its contribution on the carbon cycle must be understood. As far as we know, none study has evaluated the metabolic balance of planktonic communities in the southeastern Brazilian shelf sector and observe its role on the carbon budget. Evaluate the metabolic balance of planktonic communities in this continental shelf region is fundamental to understand and evaluate how climate change affects the metabolic balance of this three continental sectors. The main goal of this project is to evaluate the metabolism of planktonic communities in the southeastern Brazilian shelf and determine its role on the carbon budget. Indeed, we will propose to identify the planktonic metabolic differences between the three continental sectors characterizing the southeastern Brazilian shelf, to evaluate the seasonal variation of the metabolic balance in thesethree sectors and finally, to determine the role of the metabolic balance on the carbon budget in the southeastern Brazilian shelf.
  • Metodologia:
    Planktonic metabolism (GPP, CR and NCP) will be estimated through the euphotic zone (3-4 sampled depths). To evaluate the planktonic metabolism, different methods will be used concurrently. The 13C method (Slawyk et al. 1977), the 18O method (Grande 1982; Bender et al. 1987) and the dark/light method (Carpenter 1965; Carrit and Carpenter 1966) will be used as incubation methods and the bio-optical method (Gorbunov and Falkowski 2005) and the O2:Ar method will be used as free-incubation methods. For the use of 18O and O2:Ar methods and its analyses, we will collaborate with Dr. Nicolas Cassar from Duke University (N. Carolina, USA). He is an expert on the use of 18O and O2:Ar methods to measure marine planktonic metabolism and has already the required materials for sampling and analyses. This collaboration will allow to compare concurrently different methods and to determine with more accuracy the metabolic balance of the planktonic community in this coastal region. For the incubation methods, the bottles will be incubated on deck at in situ temperature and adjusting the incident natural light to that received in situ using neutral density screens. The 13C, 18O and dark/light methods are subject to the potential bottle effect. Nevertheless, for those methods, quartz bottles will be used during the incubation, letting pass through the UV radiation.
    Furthermore, bacterial abundance will be evaluated by flow cytometry upon staining with SYBRGreen I (Gasol and del Giorgio 2000). Downwelling irradiance and incident irradiance will be measured in the PAR, UV-A and UV-B using narrow bands radiometer. Dissolved organic matter (DOM) fluorescence will be evaluated at different excitation/emission couples with a spectrofluorometer (Obernosterer et al. 1999) to quantify and characterize CDOM. Nutrient (silicate, phosphate and nitrate+nitrite) and chlorophyll concentrations will be measured. We propose also to measure the variation of O2.
  • Etapas e cronograma: We plan so to sample seawater aboard scientific vessel at three different sites (Fig. 1): the north of São Sebastião Island (NSSI), the channel of São Sebastião Island (CSSI) and the south of São Sebastião Island (SSSI). Sampling at those three different sites will allow to evaluate the influence of three distinct, physically and biologically, ecosystems: the NSSI under land-derived organic matter inputs, the SSSI characterized by upwelling and finally, the CSSI characterized by strong wind fields and water current. 
    Due to the proximity of CEBIMar from CSSI site, sampling will be done monthly at the CSSI site in order to have an annual estimation of the metabolic balance and environmental parameters. We will be able so to collaborate to the ARAÇA project whose aim is to study the CSSI site and the influence of episodically continental shelf waters inputs on its ecosystem. I will collaborate with PhD student and post-doctoral fellow of the ARAÇA project working on photochemical responses of the phytoplankton community and zooplankton grazing experiments respectively. The annual sampling at the CSSI site will be done during the first two years of the fellowship.
    Sampling at the NSSI and SSSI will be made at two different periods of the second year of the fellowship: the spring/early summer bloom and the autumn/winter when the rainfalls are stronger and continental shelf water inputs are higher. Two seasonal cruises will conduct from the coastline to the continental shelf break off Cananeia and Cabo Frio respectively. Each cruise will take around one week.
    The whole duration of the project will be 36 months allowing time for implementation and completion of experimental work, analyses and writing of manuscripts. Dr Regaudie de Gioux will dedicate 100% of her time to this project. The work plan allows step-by-step goal achievement.
  • Palavras-chave: brasilian coastal planktonic metabolism  
  • Condições ambientais: Nenhuma condição especial ;   
  • Área necessária no laboratório: We will need during the samples analysis some space in the "Laboratorio Aquarela group". 
  • Equipamentos de laboratório: Automatic oceanographic/Meteorological stations;Potentiometric electrod and automated endpont detection (Titrator);Fluorometer;Flow cytometer;Spectrofluorometer;Oxygen meter and moored fluorimeter. 
  • Será necessário: Colocação ou fundeio de alguma estrutura no mar ;  Auxílio técnico para manutenção de estruturas ou material biológico na ausência dos participantes do projeto ;  Utilização de embarcação do CEBIMar ;   

  • Outros serviços de laboratório: For the moment, no. 
  • Organismos a serem coletados: Planktonic community 
  • Locais para coleta: NSSI, CSSI and SSSI 
  • Tipos de resíduos químicos e/ou infectantes a serem gerados durante o projeto:  
  • Quantidade de cada tipo de resíduo:
  • Periodicidade aproximada da geração dos resíduo:
  • Instituição(ções) de origem do projeto:

    • USP. Centro de Biologia Marinha   
  • Participante(s) do projeto:

    • Nome: Gabriela Torres  
    • Função no projeto: Outra função 
    • Início das atividades no projeto: 10/02/2015  
    • Fim das atividades no projeto: 17/07/2017  
    • Nome: Luiz Gimenes Noya  
    • Função no projeto: Outra função 
    • Início das atividades no projeto: 10/02/2015  
    • Fim das atividades no projeto: 17/07/2017  
    • Nome: Stuart Jenkins  
    • Função no projeto: Outra função 
    • Início das atividades no projeto: 10/02/2015  
    • Fim das atividades no projeto: 17/07/2017  
  • Recurso(s) destinado(s) ao projeto:

    • Situação: Concedido 
    • Agência de fomento: CNPq 
    • Tipo de recurso: Bolsa 
    • Especificar o tipo de recurso: Ciências sem Fronteiras Pos-doutorado 
    • Recursos em nome de: Aurore Regaudie-de-Gioux 
    • Recursos destinados ao CEBIMar após o término do projeto: Recursos de material de consumo em nome de Aurea Ciotti

    Produção(ões) bibliográfica(s) gerada(s) pelo projeto:

    Total de produções bibliográficas: 0

  • Data de cadastro do projeto: 16/07/2014  
  • Data da última modificação: 15/09/2014  
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