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Projeto de pesquisa

  • Pesquisa Aplicada em Holografia para Análise de Imagens do Plankton
    Holographic Applied Research for Plankton Image Analysis (HARPIA)

  • Coordenador do projeto: Rubens Mendes Lopes  
  • Autor ou executor principal do projeto: Filippo Giovanni Ghiglieno  
  • Número do projeto: 809  
  • Categoria: Pós-doutorado 
  • Data de início das atividades no CEBIMar: 01/05/2012  
  • Data de término das atividades no CEBIMar: 30/04/2015  
  • Resumo: Digital holography represents an increasingly promising approach for underwater applications. Competitive technological solutions arising from novel interdisciplinary studies are progressively paving the way for digital holography as a competitive tool for the enumeration and identification of particles and microscopic organisms in large-volume water samples. However, a challenging problem still preventing a widespread use of digital holography in fluidic applications is the massive amount of information generated by minute-range video sequences. The main goal of the HARPIA project is to develop the first software capable of processing large holographic image datasets in near-real time. At the moment no commercial product can meet these requirements. Merging the know-how of the Oceanographic Institute, University of São Paulo (LAPS/IOUSP). Worldwide computational efforts are related to reduce both the time-machine for speeding, on advanced optical components, with computer science solutions to be developed in the
    HARPIA project, we will implement integrated software solutions for the identification, measurement and enumeration of marine organisms and suspended particles. Computational solutions will include autofocus routines, search learning algorithms, a robust database, and 3D velocity measurements. Tracking trajectories and automatic holographic reconstruction tools will allow us to acquire unique data both in laboratory observations and in natural and industrial environments. Such approach will provide information on a wide range of processes, from predator-prey interactions in dense particle suspensions to the detection of tiny organisms and particles in fast-flowing systems characterized by turbulent boundary layers such as in a ballast water discharge line on board commercial ships. The developed system will be architecturally modular to meet specific application demands.
  • Metodologia: During all the HARPIA project we will work on the fundamental property of the holography: an holographically stored image is the photographically or otherwise recorded interference pattern between a wave field scattered from the object and a coherent background named reference wave. It is usually recorded on a surface, but contains the information about the entire three-dimensional wave field. This information is coded in form of interference stripes, usually not visible for the human eye due to the high spatial frequencies. The reconstruction software for the holographic image is the decoder to access at the three dimensional nature of the image. We will implement a software interface for visualizing and processing the state of the art reconstructed images coming from the hardware setup at the Laboratory of Plankton Systems, Oceanographic Institute, University of São Paulo (LAPS/IOUSP). Worldwide computational efforts are related to reduce both the time-machine for speeding up the processing (approaching as much as possible to the real-time visualization) and the percentage error in image recognition. It is challenging today to find the best algorithm to compensate the temporal gap between the capture process of the image and the numerical reconstruction.
    Different strategies can be adopted in relation to the context where the scanning holography microscopy is used.
  • Etapas e cronograma: Months 1-3: set-up in-line holographic system installation and initial database acquisition/classification
    Months 4-6: the first release of the software for the research investigation
    Months 7-11: second release of the software - the autofocus
    Months 12-17: third release of the software - the pattern recognition
    Months 18-24: fourth release of the software - particle tracking
  • Palavras-chave: Ecology, Applied Optics, Imaging, Holography, Digital Holography, Plankton, Particle Detection and Sensoring  
  • Condições ambientais: Nenhuma condição especial ;   
  • Área necessária no laboratório: Laboratory of Plankton Systems (LAPS/IOUSP) 
  • Equipamentos de laboratório: - Optical passive components (lenses, objectives, posts, holders, pinholes, spatial filters, filters) - Optical active components (lasers, LEDS) - Digital recording cameras - Acquaria - Microscope 
  • Será necessário: Coleta antecipada de organismos ;   

  • Outros serviços de laboratório: - Digital phase microscopy - FlowCam 
  • Organismos a serem coletados: Zooplankton, Phytoplakton 
  • Locais para coleta: CEBIMar, Terminal Marítimo Almirante Barroso (TEBAR) 
  • Tipos de resíduos químicos e/ou infectantes a serem gerados durante o projeto:  
  • Quantidade de cada tipo de resíduo:
  • Periodicidade aproximada da geração dos resíduo:
  • Instituição(ções) de origem do projeto:

    • USP. Instituto Oceanográfico   
  • Participante(s) do projeto:

    • Nome: Bruno Monti Oliveira  
    • Função no projeto: Graduando 
    • Início das atividades no projeto: 01/02/2013  
    • Fim das atividades no projeto: 01/02/2014  
    • Observações: Trabalho de Graduação (conclusão de curso de bacharelado em Oceanografia) vinculado ao projeto.
    • Nome: Maiá Gomes Medeiros  
    • Função no projeto: Estagiário 
    • Início das atividades no projeto: 14/01/2013  
    • Fim das atividades no projeto: 01/02/2014  
  • Recurso(s) destinado(s) ao projeto:

    • Situação: Concedido 
    • Agência de fomento: Fapesp 
    • Tipo de recurso: Bolsa 
    • Especificar o tipo de recurso: Bolsa de Pós-Doutorado - Filippo Ghiglieno 
    • Recursos em nome de: Filippo Giovanni Ghiglieno 

    Produção(ões) bibliográfica(s) gerada(s) pelo projeto:

    Total de produções bibliográficas: 0

  • Data de cadastro do projeto: 12/09/2012  
  • Data da última modificação: 19/04/2013  
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